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Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptides corresponding to ADORA2B (adenosine A2b receptor) The peptide sequence was selected form the C terminal of ADORA2B. Peptide sequence AKSLAMIVGIFALCWLPVHAVNCVTLFQPAQGKNKPKWAMNMAILLSHAN. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for Adenosine A2b R Antibody - BSA Free
Western Blot: Adenosine A2b R Antibody [NBP1-68953]
Western Blot: Adenosine A2b R Antibody [NBP1-68953] - Human breast cancer cell lines SUM149 and SUM159 were exposed to either 20% or 1% of oxygen and the expression of ADORA2B was detected by Western blot. Image from verified customer review.Western Blot: Adenosine A2b R Antibody [NBP1-68953]
Western Blot: Adenosine A2b R Antibody [NBP1-68953] - Antibody at 0.2-1 ug/mL.Applications for Adenosine A2b R Antibody - BSA Free
Application
Recommended Usage
Western Blot
1.0 ug/ml
Application Notes
Recommended positive control: 293T cell lysate.
Reviewed Applications
Read 1 review rated 4 using NBP1-68953 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Adenosine A2b R
Long Name
Adenosine A2b Receptor
Alternate Names
Adenosine A2bR, ADORA2B
Gene Symbol
ADORA2B
OMIM
NP_000667 (Human)
UniProt
Additional Adenosine A2b R Products
Product Documents for Adenosine A2b R Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Adenosine A2b R Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Adenosine A2b R Antibody - BSA Free (1)
4 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: Human breast cancer cell linesSpecies: HumanVerified Customer | Posted 12/02/2018Human breast cancer cell lines SUM149 and SUM159 were exposed to either 20% or 1% of oxygen and the expression of ADORA2B was detected through Western blot.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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