Adenylosuccinate Lyase Antibody (OTI2D10)

Novus Biologicals | Catalog # NBP2-03107

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D10
View all Adenylosuccinate Lyase Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ADSL (NP_000017) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenylosuccinate Lyase Antibody (OTI2D10)

Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Adenylosuccinate-Lyase-Antibody-OTI2D10-Western-Blot-NBP2-03107-img0012.jpg
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human lymphoma tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Flow Cytometry: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Flow Cytometry: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Flow Cytometry: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Adenylosuccinate Lyase antibody, and then analyzed by flow cytometry.
Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adenylosuccinate Lyase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adenylosuccinate Lyase.
Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Adenylosuccinate Lyase monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human Kidney tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107]

Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human liver tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Adenylosuccinate Lyase Antibody (OTI2D10)

Adenylosuccinate Lyase Antibody (OTI2D10)[NBP2-03107]

Adenylosuccinate Lyase Antibody (OTI2D10)[NBP2-03107]Simple Western� analysis of endogenous protein RNH1 from HELA lysates (0.5 mg/mL) using RNH1 Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for Adenylosuccinate Lyase Antibody (OTI2D10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

Reported in scientific publication (PMID: 32439803)

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.55 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Adenylosuccinate Lyase

Adenylsuccinate lyase is involved in both de novo synthesis of purines and formation of adenosine monophosphate from inosine monophosphate. It catalyzes two reactions in AMP biosynthesis: the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and removal of fumarate from adenylosuccinate to give AMP. Adenylosuccinase deficiency results in succinylpurinemic autism, psychomotor retardation, and, in some cases, growth retardation associated with muscle wasting and epilepsy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Alternate Names

Adenylosuccinase, adenylosuccinate lyase, AMPS, ASASE, ASL, EC 4.3.2, EC 4.3.2.2

Entrez Gene IDs

158 (Human)

Gene Symbol

ADSL

Additional Adenylosuccinate Lyase Products

Product Documents for Adenylosuccinate Lyase Antibody (OTI2D10)

Certificate of Analysis

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Product Specific Notices for Adenylosuccinate Lyase Antibody (OTI2D10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Adenylosuccinate Lyase Antibody (OTI2D10)

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Protocols

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FAQs

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