Human AFAP ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-66655

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL
View all AFAP ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human AFAP1 in samples. No significant cross-reactivity or interference between Human AFAP1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.36%

Inter-Assay Precision (Precision between assays) CV% 5.25%

Recovery for Human AFAP ELISA Kit (Colorimetric)

Recovery

86-105%

Linearity

Human AFAP ELISA Kit (Colorimetric)

Scientific Data Images for Human AFAP ELISA Kit (Colorimetric)

Human AFAP ELISA Kit (Colorimetric)

ELISA: Human AFAP ELISA Kit (Colorimetric) [NBP2-66655] -

ELISA: Human AFAP ELISA Kit (Colorimetric) [NBP2-66655] - Standard Curve Reference

Kit Contents for Human AFAP ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: AFAP

AFAP, or Actin filament-associated protein 1, contains a 81 kDa and a 90 kDa isoform, and is involved in the manipulation of actin filaments to form network or bundle structures and the regulation of cell-matrix linkage. Disease research is currently being conducted with AFAP and its relation to familial adenomatous polyposis, adenocarcinoma, breast cancer, prostate adenocarcinoma, prostatitis, polyposis, prostate cancer, and carcinoma. This protein interacts with PRKCB, PRKCD, PRKCG, ACTC1, and PRKCZ during the processes of signal transduction regulation and mechanical stimulus response.

Long Name

Actin Filament-associated Protein, 110 kDa

Alternate Names

AFAP-110, AFAP1

Gene Symbol

AFAP1

Additional AFAP Products

Product Documents for Human AFAP ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human AFAP ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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