Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP2-66674

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Urine, Milk, Cell Supernatant, Tissue Homogenate (100 uL)

Sensitivity

7.50 pg/mL

Assay Range

12.50 - 800 pg/mL
View all AIF-1/Iba1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

No significant cross-reactivity or interference between Human AIF1 and analogues was observed for this Human AIF-1/Iba1 ELISA Kit (Chemiluminescence).

Precision

Intra-Assay Precision (Precision within an assay) %CV 9.96

Inter-Assay Precision (Precision between assays) %CV 9.6

Recovery for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)

Recovery

85 - 115%

Linearity

Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)

Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)

ELISA: Human AIF-1/Iba1 ELISA Kit (Chemiluminescence) [NBP2-66674] -

ELISA: Human AIF-1/Iba1 ELISA Kit (Chemiluminescence) [NBP2-66674] - Standard Curve Reference

Kit Contents for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Antibody (100X)
  • Biotinylated Detection Antibody Diluent
  • HRP Conjugate (100X)
  • HRP Conjugate Diluent
  • Microplate
  • Plate Sealer
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B
  • Wash Buffer (25X)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: AIF-1/Iba1

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and IFN-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T.,... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Long Name

Allograft Inflammatory Factor 1

Alternate Names

AIF1, IBA1, IRT1

Gene Symbol

AIF1

Additional AIF-1/Iba1 Products

Product Documents for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)

Certificate of Analysis

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Product Specific Notices for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human AIF-1/Iba1 ELISA Kit (Chemiluminescence)

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