AIF [p Ser116] Antibody

Novus Biologicals | Catalog # NBP3-23346

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all AIF Primary Antibodies »

Product Specifications

Immunogen

Phospho-AIF (Ser-116) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-116 in human AIF. This site is conserved in rat and mouse AIF, but the amino acids surrounding the site are not well conserved. The site is not found in AIFM2 and AIFM3. [UniProt# O95831]

Modification

p Ser116

Specificity

The antibody was cross adsorbed to unphosphorylated AIF (Ser-116) peptide before affinity purification using phospho-AIF (Ser-116) peptide. This antibody antibody detects a triplet at 66, 62, and 57 kDa protein on SDS-PAGE immunoblots of human Jurkat cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment to dephosphorylated AIF.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for AIF [p Ser116] Antibody

AIF [p Ser116] Antibody

Western Blot: AIF [p Ser116] Antibody [NBP3-23346] -

Western Blot: AIF [p Ser116] Antibody [NBP3-23346] - Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIF (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIF (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).

Applications for AIF [p Ser116] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: AIF

AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Long Name

Apoptosis Inducing Factor

Alternate Names

AIFM1, COXPD6, PDCD8

Gene Symbol

AIFM1

Additional AIF Products

Product Documents for AIF [p Ser116] Antibody

Certificate of Analysis

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Product Specific Notices for AIF [p Ser116] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail