Human AIF ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP2-66673

Novus Biologicals
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL
View all AIF ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

This kit recognizes Human AIF in samples. No significant cross-reactivity or interference between Human AIF and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 8.87%

Inter-Assay Precision (Precision between assays) CV% 9.79%

Recovery for Human AIF ELISA Kit (Chemiluminescence)

Recovery

85-104%

Linearity

Human AIF ELISA Kit (Chemiluminescence)

Scientific Data Images for Human AIF ELISA Kit (Chemiluminescence)

Human AIF ELISA Kit (Chemiluminescence)

ELISA: Human AIF ELISA Kit (Chemiluminescence) [NBP2-66673] -

ELISA: Human AIF ELISA Kit (Chemiluminescence) [NBP2-66673] - Standard Curve Reference

Kit Contents for Human AIF ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: AIF

AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Long Name

Apoptosis Inducing Factor

Alternate Names

AIFM1, COXPD6, PDCD8

Gene Symbol

AIFM1

Additional AIF Products

Product Documents for Human AIF ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human AIF ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Customer Reviews for Human AIF ELISA Kit (Chemiluminescence)

There are currently no reviews for this product. Be the first to review Human AIF ELISA Kit (Chemiluminescence) and earn rewards!

Have you used Human AIF ELISA Kit (Chemiluminescence)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits

Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail