Q: For my project I'm interested in an primary antibody that is not currently available on the market. The protein I'm interested in is aryl hydrocarbon receptor interacting protein. I would like to use the antibody for Western Blot and/or Immunoprecipitation, and this antibody has to be specific for a zebra fish protein. I would like to ask for a price quote.

A: We currently have no AIPL1 antibodies validated in Zebra fish. If you are interested in testing in Zebra fish, you would qualify for our Innovators Reward Program. You may also contact us at innovators@novusbio.com should you have any questions about the Innovators Reward Program.

AIPL1 Overexpression Lysate

Novus Biologicals | Catalog # NBL1-07416

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for AIPL1 Overexpression Lysate

AIPL1 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC204079

Accession#: NM_014336

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

43.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for AIPL1 Overexpression Lysate

Western Blot: AIPL1 Overexpression Lysate [NBL1-07416]

Western Blot: AIPL1 Overexpression Lysate (Adult Normal) [NBL1-07416] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AIPL1.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: AIPL1

Leber congenital amaurosis (LCA) accounts for at least 5% of all inherited retinal disease and is the most severe inherited retinopathy with the earliest age of onset. Individuals affected with LCA are diagnosed at birth or in the first few months of life with severely impaired vision or blindness, nystagmus and an abnormal or flat electroretinogram. The photoreceptor/pineal -expressed gene, AIPL1, encoding aryl-hydrocarbon interacting protein-like 1, was mapped within the LCA4 candidate region. The protein contains three tetratricopeptide motifs, consistent with nuclear transport or chaperone activity. AIPL1 mutations may cause approximately 20% of recessive LCA. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

AIPL2, aryl hydrocarbon receptor interacting protein-like 1, aryl hydrocarbon receptor-interacting protein-like 1, aryl-hydrocarbon-interacting protein-like 1, LCA4

Gene Symbol

AIPL1

Additional AIPL1 Products

Product Documents for AIPL1 Overexpression Lysate

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Certificate of Analysis

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Product Specific Notices for AIPL1 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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