AKAP2 Antibody

Novus Biologicals | Catalog # NB100-61603

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all AKAP2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human A kinase (PRKA) anchor protein 2 using the numbering given in entry Q9Y2D5.2 (GeneID 11217).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKAP2 Antibody

Western Blot: AKAP2 Antibody [NB100-61603]

Western Blot: AKAP2 Antibody [NB100-61603]

Western Blot: AKAP2 Antibody [NB100-61603] - Detection of Human AKAP2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AKAP2 antibody NB100-61603 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: AKAP2 Antibody [NB100-61603]

Immunoprecipitation: AKAP2 Antibody [NB100-61603]

Immunoprecipitation: AKAP2 Antibody [NB100-61603] - Detection of human AKAP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AKAP2 antibody NB100-61603 (lot 2) used for IP at 6 ug per reaction. AKAP2 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated AKAP2, NB100-61603 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for AKAP2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 -10 ug/mg lysate

Western Blot

1:200-1:1000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AKAP2

AKAP2 (A-kinase anchoring protein 2) was identified in a yeast two-hybrid screen as a protein that interacts with protein kinase A (PKA). AKAP2 functions to direct the subcellular localization of PKA and may also function as a link for cytoskeletal signaling complexes. Structurally, AKAP2 contains two tandem RGS domains, a PKA-binding motif, and a PDZ (PSD95/Dlg/ZO1)-binding motif.

Alternate Names

A kinase (PRKA) anchor protein 2, AKAP-2, FLJ53197, protein kinase A2

Entrez Gene IDs

11217 (Human)

Gene Symbol

PALM2AKAP2

UniProt

Q9Y2D5.2 (Human)

Additional AKAP2 Products

Product Documents for AKAP2 Antibody

Certificate of Analysis

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Product Specific Notices for AKAP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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