Akti-1/2

Tocris Bioscience | Catalog # 5773

Potent and selective dual Akt1 and 2 inhibitor
Tocris Bioscience

Key Product Details

Description

Potent and selective dual Akt1 and 2 inhibitor

Alternative Names

Akt Inhibitor VIII
View all Akt (Protein Kinase B) Inhibitors »

Product Description

Akti-1/2 is a potent and selective dual Akt1 and 2 inhibitor (IC50 values are 50 and 210 nM, respectively). Selective for Akt1 and 2 over a panel of other tyrosine and serine/threonine kinases. Sensitizes LnCaP cells to TRAIL (TNF-related apoptosis-inducing ligand) induced apoptosis. Also enhances CAR and TCR retroviral transduction of human T cells. Active in vivo.

Product Specifications for Akti-1/2

Molecular Weight

551.64

Formula

C34H29N7O

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

1,3-Dihydro-1-[1-[[4-(6-phenyl-1H-imidazo[4,5-g]quinoxalin-7-yl)phenyl]methyl]-4-piperidinyl]-2H-benzimidazol-2-one

CAS Number

612847-09-3

PubChem ID

10196499

InChI Key

BIWGYFZAEWGBAL-UHFFFAOYSA-N

SMILES

O=C1NC2=C(C=CC=C2)N1C(CC3)CCN3CC(C=C4)=CC=C4C5=NC6=CC(N=CN7)=C7C=C6N=C5C8=CC=CC=C8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.03 20 with gentle warming

Preparing Stock Solutions for Akti-1/2

The following data is based on the product molecular weight 551.64.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 9.06 mL 45.32 mL 90.64 mL
1 mM 1.81 mL 9.06 mL 18.13 mL
2 mM 0.91 mL 4.53 mL 9.06 mL
10 mM 0.18 mL 0.91 mL 1.81 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 22 publications citing the usage of this product.

Product Documents for Akti-1/2

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Akti-1/2

For research use only

Customer Reviews for Akti-1/2 (2)

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  • Akti-1/2 blocks LPA-mediated COX-2 expression
    Name: Peter Cruz
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Osteosarcoma
    Verified Customer | Posted 12/13/2018
    Human osteosarcoma cells were incubated with 10 μM Akti-1/2 for 30 min prior to treatment with 10 μM LPA to estimate COX-2 expression using Western blot analysis. Akti-1/2 abolished LPA-induced COX-2 expression.
    Akti-1/2 5773
  • Akt-1/2 blocks 15d-PGJ2-induced Akt phosphorylation
    Name: Christopher Trummer
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: MG-63 osteosarcoma cells
    Verified Customer | Posted 10/15/2018
    MG-63 cells were incubated with PD169316 (25 μM), LY294002 (10 μM) or Akt-1/2 (5 μM Akt-I) for 30 min prior to 15d-PGJ2 treatment (20 μM) for 1 h to follow pAkt expression using Western blot. Akt-1/2 completely blocked 15d-PGJ2-induced Akt phosphorylation in MG-63 cells.
    Akti-1/2 5773

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