ALAD Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89158

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ALAD Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GCQVVAPSDMMDGRVEAIKEALMAHGLGNRVSVMSYSAKFASCFYGPFRDAAKSSPAFGDRRCYQLPPGARGLALRAVDRD

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28857503).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ALAD Antibody - BSA Free

Western Blot: ALAD Antibody [NBP1-89158]

Western Blot: ALAD Antibody [NBP1-89158]

Western Blot: ALAD Antibody [NBP1-89158] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Liver
Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158]

Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158]

Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158] - Staining in human adrenal gland and pancreas tissues using anti-ALAD antibody. Corresponding ALAD RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158]

Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158]

Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158] - Staining of human adrenal gland shows high expression.
Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158]

Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158]

Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158] - Staining of human pancreas shows low expression as expected.
Simple Western: ALAD Antibody [NBP1-89158]

Simple Western: ALAD Antibody [NBP1-89158]

Simple Western: ALAD Antibody [NBP1-89158] - Simple Western lane view shows a specific band for ALAD in 0.2 mg/ml of h. Liver (left) and h. Adrenal (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: ALAD Antibody [NBP1-89158]

Simple Western: ALAD Antibody [NBP1-89158]

Simple Western: ALAD Antibody [NBP1-89158] - Electropherogram image of the corresponding Simple Western lane view. ALAD antibody was used at 1:200 dilution on h. Liver and h. Adrenal lysate(s) respectively.
ALAD Antibody Immunohistochemistry-Paraffin: ALAD Antibody Antibody [NBP1-89158]

Immunohistochemistry-Paraffin: ALAD Antibody Antibody [NBP1-89158]

Staining of human kidney, liver, pancreas and skeletal muscle using NBP1-89158 (A) shows similar protein distribution across tissues to independent antibody NBP1-89157 (B).

Applications for ALAD Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in h. Liver and h. Adrenal lysates, separated by Size, antibody dilution of 1:200, apparent MW was 39 kDa

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALAD

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. [provided by RefSeq]

Alternate Names

ALADHEC 4.2.1.24, aminolevulinate dehydratase, aminolevulinate, delta-, dehydratase, delta-aminolevulinic acid dehydratase, PBGSMGC5057, Porphobilinogen synthase

Gene Symbol

ALAD

Additional ALAD Products

Product Documents for ALAD Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ALAD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ALAD Antibody - BSA Free

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Protocols

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