Alanyl tRNA synthetase Antibody

Novus Biologicals | Catalog # NBP3-30487

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Golden Syrian Hamster (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Alanyl tRNA synthetase Primary Antibodies »

Product Specifications

Immunogen

between 275 and 325

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alanyl tRNA synthetase Antibody

Alanyl tRNA synthetase Antibody

Western Blot:[NBP3-30487]-

Western Blot:[NBP3-30487]-Detection of human and mouse AARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-AARS antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. AARS was also immunoprecipitated by rabbit anti-AARS antibodies and, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes and 30 seconds.

Applications for Alanyl tRNA synthetase Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 µg/mg lysate

Western Blot

1:2,000 - 1:10,000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-buffered Saline 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Alanyl tRNA synthetase

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq]

Alternate Names

alanine tRNA ligase 1, cytoplasmic, Alanine--tRNA ligase, alanyl-tRNA synthetase, alanyl-tRNA synthetase, cytoplasmic, alaRS, CMT2N, EC 6.1.1, EC 6.1.1.7, Renal carcinoma antigen NY-REN-42

Gene Symbol

AARS1

Additional Alanyl tRNA synthetase Products

Product Documents for Alanyl tRNA synthetase Antibody

Certificate of Analysis

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Product Specific Notices for Alanyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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