Alas1 Antibody (2K1G3)

Novus Biologicals | Catalog # NBP3-16487

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2K1G3 expressed in HEK293
View all Alas1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 541-640 of human ALAS1 (P13196). TEVCDELMSRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNYFLENLLVTWKQVGLELKPHSSAECNFCRRPLHFEVMSEREKSYFSGLSKLVSAQA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alas1 Antibody (2K1G3)

Western Blot: Alas1 Antibody (2K1G3) [NBP3-16487]

Western Blot: Alas1 Antibody (2K1G3) [NBP3-16487]

Western Blot: Alas1 Antibody (2K1G3) [NBP3-16487] - Western blot analysis of extracts of various cell lines, using Alas1 Rabbit mAb (NBP3-16487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Applications for Alas1 Antibody (2K1G3)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Alas1

Delta-aminolevulinate synthase (ALAS; EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2 (MIM 301300).[supplied by OMIM]

Alternate Names

5-aminolevulinic acid synthase 1, ALAS, ALAS3, ALASH, ALAS-H, aminolevulinate, delta-, synthase 1, Delta-ALA synthase 1,5-aminolevulinate synthase, nonspecific, mitochondrial, Delta-aminolevulinate synthase 1, EC 2.3.1, EC 2.3.1.37, MIG4, migration-inducing protein 4

Gene Symbol

ALAS1

Additional Alas1 Products

Product Documents for Alas1 Antibody (2K1G3)

Certificate of Analysis

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Product Specific Notices for Alas1 Antibody (2K1G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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