alcohol dehydrogenase Antibody

Novus Biologicals | Catalog # NB300-956

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all alcohol dehydrogenase Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence STAGKVMKCKA corresponding to N-Terminus according to NP_000658.1, NP_000659.2, NP_000660.1.

Specificity

This antibody is expected to recognise the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of human alcohol dehydrogenase.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for alcohol dehydrogenase Antibody

Western Blot: alcohol dehydrogenase Antibody [NB300-956]

Western Blot: alcohol dehydrogenase Antibody [NB300-956]

Western Blot: alcohol dehydrogenase Antibody [NB300-956] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: alcohol dehydrogenase Antibody [NB300-956]

Western Blot: alcohol dehydrogenase Antibody [NB300-956]

Western Blot: alcohol dehydrogenase Antibody [NB300-956] - Analysis of pan alcohol dehydrogenase in Human Liver lysate

Applications for alcohol dehydrogenase Antibody

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:10-1:500

Peptide ELISA

detection limit 1:8000

Western Blot

1-3 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in human liver lysates (calculated MW of 39.9 kDa band according to NP_000658.1, NP_000659.2 and 000660.1). IHC: An anonymous customer has reported on human liver.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alcohol dehydrogenase

This gene encodes class I alcohol dehydrogenase, alpha subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. This gene is monomorphic and predominant in fetal and infant livers, whereas the genes encoding beta and gamma subunits are polymorphic and strongly expressed in adult livers.

Alternate Names

ADH, alpha subunit, ADH1alcohol dehydrogenase 1A, alcohol dehydrogenase 1 (class I), alpha polypeptide, alcohol dehydrogenase 1A (class I), alpha polypeptide, Alcohol dehydrogenase subunit alpha, aldehyde reductase, EC 1.1.1, EC 1.1.1.1

Entrez Gene IDs

124 (Human)

Gene Symbol

ADH1A

UniProt

NP_000658.1 (Human)

Additional alcohol dehydrogenase Products

Product Documents for alcohol dehydrogenase Antibody

Certificate of Analysis

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Product Specific Notices for alcohol dehydrogenase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alcohol dehydrogenase Antibody

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Protocols

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