Aldosterone Parameter Assay Kit

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KGE016 has been discontinued.
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Multi-species Aldosterone ELISA Standard Curve
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Product Details
Citations (10)

Aldosterone Parameter Assay Kit Summary

96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (34 uL), Serum (34 uL), EDTA Plasma (34 uL), Heparin Plasma (34 uL), Urine (10 uL)
22.4 pg/mL
Assay Range
24.7 - 6,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Measures Aldosterone levels in various samples
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Parameter Aldosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Aldosterone in cell culture supernates, serum, plasma, and urine.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 278 878 2536 263 832 2419
Standard Deviation 9.49 17.3 34.4 31.7 83 230
CV% 3.4 2 1.4 12.1 10 9.5


The recovery of Aldosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 89-99
EDTA Plasma (n=4) 89 76-110
Heparin Plasma (n=4) 89 79-107
Serum (n=4) 88 78-108
Urine (n=4) 101 95-106


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Aldosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay as directed in the Sample Preparation section.
Multi-species Aldosterone ELISA Linearity

Scientific Data

Multi-species Aldosterone ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Aldosterone Primary Antibody Solution
  4.   Add 100 µL of Aldosterone Primary Antibody Solution to each well (excluding the NSB wells). Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  6. 50 µL Assay Diluent RD1-120
  7.   Add 50 µL of Assay Diluent RD1-120 to each well.

  8. 75 µL Standard, Control, or Sample
  9.   Add 75 µL of Standard, control, or sample to the appropriate wells.

  10. 75 µL Calibrator Diluent RD5-69
  11.   Add 75 µL of Calibrator Diluent RD5-69 to the zero standard (B0) wells and NSB wells.

  12. 50 µL Aldosterone Conjugate
  13.   Add 50 µL of Aldosterone Conjugate to all wells. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  14.   Aspirate and wash 4 times.

  15. 200 µL Substrate Solution
  16.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  17. 100 µL Stop Solution
  18.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Aldosterone Parameter Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Low sodium intake ameliorates hypertension and left ventricular hypertrophy in mice with primary aldosteronism
    Authors: Z Wang, X Zhao, L Bu, K Liu, Z Li, H Zhang, X Zhang, F Yuan, S Wang, Z Guo, L Shi
    Frontiers in Physiology, 2023-02-15;14(0):1136574.
    Species: Mouse
    Sample Types: Plasma
  2. Unravelling the sex-specific diversity and functions of adrenal gland macrophages
    Authors: B Dolfi, A Gallerand, MM Firulyova, Y Xu, J Merlin, A Dumont, A Castiglion, N Vaillant, S Quemener, H Gerke, MI Stunault, PR Schrank, SH Kim, A Zhu, J Ding, J Gilleron, V Magnone, P Barbry, D Dombrowicz, C Duranton, A Wakkach, C Blin-Wakka, B Becher, S Pagnotta, RJ Argüello, P Rantakari, S Chakarov, F Ginhoux, K Zaitsev, KW Kim, L Yvan-Charv, RR Guinamard, JW Williams, S Ivanov
    Cell Reports, 2022-06-14;39(11):110949.
    Species: Mouse
    Sample Types: Serum, Tissue Homogenates
  3. Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum
    Authors: A Khokhlova, T Myachina, D Volzhanino, X Butova, A Kochurova, V Berg, I Gette, G Moroz, S Klinova, I Minigaliev, O Solovyova, I Danilova, K Sokolova, G Kopylova, D Shchepkin
    International Journal of Molecular Sciences, 2022-02-02;23(3):.
    Species: Rat
    Sample Types: Plasma
  4. The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males.
    Authors: Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K
    Antioxidants (Basel), 2021-05-28;10(6):.
    Species: Human
    Sample Types: Urine
  5. Neuronal aldosterone elicits a distinct genomic response in pain signaling molecules contributing to inflammatory pain
    Authors: M Shaqura, L Li, DM Mohamed, X Li, S Treskatsch, C Buhrmann, M Shakibaei, A Beyer, SA Mousa, M Schäfer
    J Neuroinflammation, 2020-06-12;17(1):183.
    Species: Rat
    Sample Types: Cell Lysates
  6. Intradialytic hypotension: beyond hemodynamics.
    Authors: Hajal J, Joubran N, Sleilaty G, Chacra D, Saliba Y, Assaad S, Chelala D, Fares N
    Physiol Res, 2019-08-19;68(5):793-805.
    Species: Human
    Sample Types: Plasma
  7. Age-dependent impairment of the erythropoietin response to reduced central venous pressure in HFpEF patients
    Authors: D Montero, T Haider, J Barthelmes, JP Goetze, S Cantatore, C Lundby, I Sudano, F Ruschitzka, AJ Flammer
    Physiol Rep, 2019-03-01;7(5):e14021.
    Species: Human
    Sample Types: Plasma
  8. Danhong injection reduces vascular remodeling and up-regulates the Kallikrein-kinin system in spontaneously hypertensive rats
    Authors: X Yang, J Orgah, D Wang, G Fan, H Jingyang, J Han, G Qin, X Gao, Y Zhu
    Sci Rep, 2017-06-27;7(1):4308.
    Species: Rat
    Sample Types: Serum
  9. Hydrochlorothiazide modulates ischemic heart failure-induced cardiac remodeling via inhibiting angiotensin II type 1 receptor pathway in rats
    Authors: Jinghong Luo
    Cardiovasc Ther, 2017-04-01;0(0):.
    Species: Human
    Sample Types: Plasma
  10. Protocol for a prospective, controlled, observational study to evaluate the influence of hypoxia on healthy volunteers and patients with inflammatory bowel disease: the Altitude IBD Study
    Authors: S Vavricka, PA Ruiz, S Scharl, L Biedermann, M Scharl, C de ValliŠr, C Lundby, RH Wenger, L Held, TM Merz, M Gassmann, T Lutz, A Kunz, D Bron, A Fontana, L Strauss, A Weber, M Fried, G Rogler, J Zeitz
    BMJ Open, 2017-01-05;7(1):e013477.
    Species: Human
    Sample Types: Plasma


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