Aldosterone Parameter Assay Kit

R&D Systems | Catalog # KGE016

R&D Systems
Discontinued Product
KGE016 has been discontinued. View all Aldosterone products.

Key Product Details

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (34 µL), Serum (34 µL), EDTA Plasma (34 µL), Heparin Plasma (34 µL), Urine (10 µL)

Sensitivity

22.4 pg/mL

Assay Range

24.7-6000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
View all Aldosterone ELISA Kits »

Product Summary for Aldosterone Parameter Assay Kit

The Parameter Aldosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Aldosterone in cell culture supernates, serum, plasma, and urine.

Product Specifications

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

Biotin

Species

Multi-Species

Specificity

Measures Aldosterone levels in various samples

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 278 878 2536 263 832 2419
Standard Deviation 9.49 17.3 34.4 31.7 83.0 230
CV% 3.4 2.0 1.4 12.1 10.0 9.5

Recovery for Aldosterone Parameter Assay Kit

The recovery of Aldosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 89-99
EDTA Plasma (n=4) 89 76-110
Heparin Plasma (n=4) 89 79-107
Serum (n=4) 88 78-108
Urine (n=4) 101 95-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Aldosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay as directed in the Sample Preparation section.

Multi-species Aldosterone ELISA Linearity

Scientific Data Images for Aldosterone Parameter Assay Kit

Multi-species Aldosterone ELISA Standard Curve

Multi-species Aldosterone ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Aldosterone

Aldosterone is a steroid hormone (mineralocorticoid family) produced by the outer-section (zona glomerulosa) of the adrenal cortex in the adrenal gland to regulate sodium and potassium balance in the blood. It is synthesized from cholesterol by aldosterone synthase, which is absent in other sections of the adrenal gland. It acts in the kidney promoting the reabsorption of sodium ions (Na+) into the blood. Water follows the salt and this helps maintain normal blood pressure. It also acts on sweat glands to reduce the loss of sodium in perspiration and taste cells to increase the sensitivity of the taste buds to sources of sodium.

Additional Aldosterone Products

Product Documents for Aldosterone Parameter Assay Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Aldosterone Parameter Assay Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Aldosterone Parameter Assay Kit

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Protocols

View specific protocols for Aldosterone Parameter Assay Kit (KGE016):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Aldosterone Primary Antibody Solution
  3.   Add 100 µL of Aldosterone Primary Antibody Solution to each well (excluding the NSB wells). Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  4.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    5. 50 µL Assay Diluent RD1-120
  5.   Add 50 µL of Assay Diluent RD1-120 to each well.
    6. 75 µL Standard, Control, or Sample
  6.   Add 75 µL of Standard, control, or sample to the appropriate wells.
    7. 75 µL Calibrator Diluent RD5-69
  7.   Add 75 µL of Calibrator Diluent RD5-69 to the zero standard (B0) wells and NSB wells.
    8. 50 µL Aldosterone Conjugate
  8.   Add 50 µL of Aldosterone Conjugate to all wells. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  9.   Aspirate and wash 4 times.
    10. 200 µL Substrate Solution
  10.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    11. 100 µL Stop Solution
  11.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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