Aldosterone Parameter Assay Kit

Catalog # Availability Size / Price Qty
KGE016
Control Products Available
Multi-species Aldosterone Standard Curve
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Product Details
Procedure
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Aldosterone Parameter Assay Kit Summary

Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (34 uL), Serum (34 uL), EDTA Plasma (34 uL), Heparin Plasma (34 uL), Urine (10 uL)
Sensitivity
22.4 pg/mL
Assay Range
24.7 - 6,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Measures Aldosterone levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Parameter Aldosterone assay is a 3.5 hour competitive enzyme immunoassay designed to measure Aldosterone in cell culture supernates, serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 278 878 2536 263 832 2419
Standard Deviation 9.49 17.3 34.4 31.7 83 230
CV% 3.4 2 1.4 12.1 10 9.5

Recovery

The recovery of Aldosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 89-99
EDTA Plasma (n=4) 89 76-110
Heparin Plasma (n=4) 89 79-107
Serum (n=4) 88 78-108
Urine (n=4) 101 95-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Aldosterone were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay as directed in the Sample Preparation section.
Multi-species Aldosterone ELISA Linearity

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Aldosterone Primary Antibody Solution
  4.   Add 100 µL of Aldosterone Primary Antibody Solution to each well (excluding the NSB wells). Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  6. 50 µL Assay Diluent RD1-120
  7.   Add 50 µL of Assay Diluent RD1-120 to each well.

  8. 75 µL Standard, Control, or Sample
  9.   Add 75 µL of Standard, control, or sample to the appropriate wells.

  10. 75 µL Calibrator Diluent RD5-69
  11.   Add 75 µL of Calibrator Diluent RD5-69 to the zero standard (B0) wells and NSB wells.

  12. 50 µL Aldosterone Conjugate
  13.   Add 50 µL of Aldosterone Conjugate to all wells. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  14.   Aspirate and wash 4 times.

  15. 200 µL Substrate Solution
  16.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  17. 100 µL Stop Solution
  18.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

FAQs

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ELISA Controls

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