Alkaline Phosphatase/ALPP Antibody (S01-8I3)

Novus Biologicals | Catalog # NBP3-20050

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S01-8I3 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide of human Alkaline Phosphatase/ALPP (Uniprot # P05187)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alkaline Phosphatase/ALPP Antibody (S01-8I3)

Western Blot: Alkaline Phosphatase/ALPP Antibody (S01-8I3) [NBP3-20050]

Western Blot: Alkaline Phosphatase/ALPP Antibody (S01-8I3) [NBP3-20050]

Western Blot: Alkaline Phosphatase/ALPP Antibody (S01-8I3) [NBP3-20050] - Western blot detection of Alkaline Phosphatase/ALPP in Hela cell lysates using NBP3-20050 (1:500 diluted). Predicted band size: 58kDa. Observed band size: 70kDa.
Western Blot: Alkaline Phosphatase/ALPP Antibody (S01-8I3) [NBP3-20050]

Western Blot: Alkaline Phosphatase/ALPP Antibody (S01-8I3) [NBP3-20050]

Western Blot: Alkaline Phosphatase/ALPP Antibody (S01-8I3) [NBP3-20050] - Western blot detection of Alkaline Phosphatase/ALPP in Hela cell lysates using NBP3-20050 (1:1000 diluted). Predicted band size: 58KDa. Observed band size: 70KDa.

Applications for Alkaline Phosphatase/ALPP Antibody (S01-8I3)

Application
Recommended Usage

Western Blot

1/2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase/ALPP

Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.

Long Name

Alkaline Phosphatase, Placental Type

Alternate Names

Alkaline Phosphatase, PLAP, SEAP

Gene Symbol

ALPP

Additional Alkaline Phosphatase/ALPP Products

Product Documents for Alkaline Phosphatase/ALPP Antibody (S01-8I3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (S01-8I3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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FAQs for Alkaline Phosphatase/ALPP Antibody (S01-8I3)

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  • Q: I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?

    A:

    None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

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