Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

Novus Biologicals | Catalog # NBP2-22193

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F4

Format

BSA Free
View all Alkaline Phosphatase, Tissue Non-Specific Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Alkaline Phosphatase, Tissue Non-Specific expressed in E. Coli.

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free (NBP2-22193) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4)BSA Free [NBP2-22193]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4)BSA Free [NBP2-22193]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193] - Analysis of Human engineered ALPL cells using Alkaline Phosphatase. Primary antibody dilution: 1:1000. Image from verified customer review.
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis of ovarian cancer tissues using Alkaline Phosphatase, Tissue Non-Specific mouse mAb with DAB staining.
Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193] - Analysis of Human engineered ALPL cells using Alkaline Phosphatase. Image from verified customer reviews.
Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4)BSA Free [NBP2-22193]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4)BSA Free [NBP2-22193]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis using Alkaline Phosphatase, Tissue Non-Specific mAb against human Alkaline Phosphatase, Tissue Non-Specific (AA: 18-502) recombinant protein. (Expected MW is 78.9 kDa)
Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4)BSA Free [NBP2-22193]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4)BSA Free [NBP2-22193]

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis using Alkaline Phosphatase, Tissue Non-Specific mouse mAb against HeLa (1), and NTERA-2 (4) cell lysate.
Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis of MCF-7 cells using Alkaline Phosphatase, Tissue Non-Specific mouse mAb (green) and negative control (red).
ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193]

ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Applications for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-22193 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Tissue Non-Specific

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.

Alternate Names

Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935

Gene Symbol

ALPL

Additional Alkaline Phosphatase, Tissue Non-Specific Products

Product Documents for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

Customer Reviews for Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free
    Name: Ming Lu
    Application: Western Blot
    Sample Tested: engineered ALPL cells
    Species: Human
    Verified Customer | Posted 09/13/2022
    NBP2-22193 is very strong in western blotting ALPL. I used 1:1000 dilution in this experiment. The image exposure time was only 0.5 second. I believe further dilution such as 1:5000 with primary antibody would be OK too.
    Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free NBP2-22193
  • Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free
    Name: Ming Lu
    Application: Flow Cytometry
    Sample Tested: engineered ALPL cells
    Species: Human
    Verified Customer | Posted 09/13/2022
    Very low background when incubated with parental cells, has a clean shift when incubated with ALPL overexpressing cells.
    Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free NBP2-22193

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies