Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free
Novus Biologicals | Catalog # NB600-540
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49)BSA Free [NB600-540]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-Western-Blot-NB600-540-img0009.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49)BSA Free [NB600-540]")
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Key Product Details
Species Reactivity
Validated:
Human, Porcine, Feline, Rabbit
Cited:
Human, Rabbit
Applications
Validated:
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot, Flow Cytometry, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # TRA-2-49
Format
BSA Free
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Product Specifications
Immunogen
2102Ep Human Embryonal Carcinoma Cells
Epitope
Liver/Bone/Kidney Alkaline Phosphatase
Reactivity Notes
Reactivity has been demonstrated with Liver/ Bone/Kidney isozyme of Alkaline Phosphatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon), New World Monkeys (Owl Monkey, Squirrel Monkey), Cat, Pig, Rabbit and Tiger.
No reactivity has been observed with Old World Monkeys (Baboon, Rhesus monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey), Bovine, Canine, Goat, Guinea Pig, Hamster, Mouse, Rat and Sheep. This antibody does not cross react with other isozymes of AP.
No reactivity has been observed with Old World Monkeys (Baboon, Rhesus monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey), Bovine, Canine, Goat, Guinea Pig, Hamster, Mouse, Rat and Sheep. This antibody does not cross react with other isozymes of AP.
Localization
Cell Membrane
Specificity
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells. This antibody does not cross react with other isozymes of AP.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images
Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49)BSA Free [NB600-540]
Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-Western-Blot-NB600-540-img0009.jpg![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-540-img0011.jpg "Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540]")
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540]
Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540] - Confocal fluorescent analysis of A431 using anti-TRA-2-49 Mouse monoclonal antibody (Red). Actin filaments have been labeled with AlexaFluor(R) 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). Positive cytosol staining.![Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-Flow-Cytometry-NB600-540-img0008.jpg "Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540]")
Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540]
Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Analysis using anti-TRA-2-49. 2102Ep cells were stained with anti-TRA-2-49 antibody (Green) or isotype control antibody (grey).
Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540] -
Comparison on osteogenic-related proteins (OCN, BMP2, COL1a1, DDk1, ALP) for osteogenic micro-tissue and digested cells by Western blot. MT, osteogenic micro-tissue; DC, digested cellsApplications
Application
Recommended Usage
Flow Cytometry
5-20 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
1:10-1:500
Application Notes
Does not work on frozen sections. Use in WB reported in scientific literature (PMID: 30486863). Use in IHC reported in scientific literature (PMID:34394354).
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
20mM PBS (pH 7.6) and 0.25M NaCl
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase, Tissue Non-Specific
Alternate Names
Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935
Gene Symbol
ALPL
Additional Alkaline Phosphatase, Tissue Non-Specific Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs
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Q: Please advice if NB600-540 Alkaline Phosphatase Antibody can be used for IHC-P ?
A:
This antibody has not yet been tested in IHC on paraffin embedded tissues. This antibody has only been validated in Flow, ICC, and IP. If you would like to try this antibody in IHC, we can offer our Innovator's Reward Program. Otherwise, we have the following products available to this target that are validated in IHC with paraffin-embedded tissues: View roducts.
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