alpha 1-Acid Glycoprotein Antibody (2F9-1F10) - Azide and BSA Free

Novus Biologicals | Catalog # H00005004-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F9-1F10

Format

Azide and BSA Free
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Product Specifications

Immunogen

ORM1 (AAH26238, 18 a.a. ~ 201 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AQIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQDQCIYNTTYLNVQRENGTISRYVGGQEHFAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES

Specificity

ORM1 - orosomucoid 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for alpha 1-Acid Glycoprotein Antibody (2F9-1F10) - Azide and BSA Free

Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody (M01), clone 2F9-1F10.Lane 1: ORM1 transfected lysate(23.5 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

Immunohistochemistry-Paraffin: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

Immunohistochemistry-Paraffin: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. Antibody concentration 1 ug/ml.
Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi ( Cat # H00005004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody (M01), clone 2F9-1F10 (Cat # H00005004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

Western Blot: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - ORM1 monoclonal antibody (M01), clone 2F9-1F10 Analysis of ORM1 expression in MCF-7.
Immunoprecipitation: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

Immunoprecipitation: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

Immunoprecipitation: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Analysis of ORM1 transfected lysate using anti-ORM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORM1 MaxPab rabbit polyclonal antibody.
ELISA: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

ELISA: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01]

ELISA: alpha 1-Acid Glycoprotein Antibody (2F9-1F10) [H00005004-M01] - Detection limit for recombinant GST tagged ORM1 is approximately 0.1ng/ml as a capture antibody.

Applications for alpha 1-Acid Glycoprotein Antibody (2F9-1F10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:1000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha 1-Acid Glycoprotein

This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression.

Alternate Names

AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid

Entrez Gene IDs

5004 (Human)

Gene Symbol

ORM1

OMIM

138600 (Human)

Additional alpha 1-Acid Glycoprotein Products

Product Documents for alpha 1-Acid Glycoprotein Antibody (2F9-1F10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for alpha 1-Acid Glycoprotein Antibody (2F9-1F10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha 1-Acid Glycoprotein Antibody (2F9-1F10) - Azide and BSA Free

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Protocols

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FAQs

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