alpha-Defensin 1 Antibody

Novus Biologicals | Catalog # NBP2-27562

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all alpha-Defensin 1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DESLAPKHPGSRKN corresponding to internal region according to NP_004075.1, NP_005208.1.

Specificity

This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for alpha-Defensin 1 Antibody

Western Blot: alpha-Defensin 1 Antibody [NBP2-27562]

Western Blot: alpha-Defensin 1 Antibody [NBP2-27562]

Western Blot: alpha-Defensin 1 Antibody [NBP2-27562] - Analysis of Human Spleen lysate (35ug protein in RIPA buffer) using NBP2-27562 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: alpha-Defensin 1 Antibody [NBP2-27562]

Immunohistochemistry-Paraffin: alpha-Defensin 1 Antibody [NBP2-27562]

Immunohistochemistry-Paraffin: alpha-Defensin 1 Antibody [NBP2-27562] - (3.8 ug/ml) Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for alpha-Defensin 1 Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 6 ug/ml

Immunohistochemistry-Paraffin

4 - 6 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 10 kDa band observed in human spleen lysates (calculated MW of 10.2 kDa band according to NP_004075.1). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Thymus shows staining of selected cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Defensin 1

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundantin the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine,respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequenceand distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8.The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likelyplays a role in phagocyte-mediated host defense. It differs from defensin, alpha 3 by only one amino acid. (providedby RefSeq)

Alternate Names

alphaDefensin 1, DEF1, DEFA1, HNP-1, HP1, MRS

Gene Symbol

DEFA1

UniProt

NP_004075.1 (Human)

Additional alpha-Defensin 1 Products

Product Documents for alpha-Defensin 1 Antibody

Certificate of Analysis

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Product Specific Notices for alpha-Defensin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Defensin 1 Antibody

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Protocols

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