AMICA/JAML Antibody (4E10) - BSA Free

Novus Biologicals | Catalog # NBP1-43309

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 4E10

Format

BSA Free
View all AMICA/JAML Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues C(267) D G I L G D N F R P T Q(279) of mouse CARP.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for AMICA/JAML Antibody (4E10) - BSA Free

Flow Cytometry: AMICA/JAML Antibody (4E10) - BSA Free [NBP1-43309]

Flow Cytometry: AMICA/JAML Antibody (4E10) - BSA Free [NBP1-43309]

Flow Cytometry: AMICA/JAML Antibody (4E10) [NBP1-43309] - Staining of mouse JAML-transfected CHO cells with 0.5 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse JAML Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were used for analysis.

Applications for AMICA/JAML Antibody (4E10) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:10-1:500
Application Notes
This 4E10 antibody has been tested by flow cytometric analysis of mouse lysed whole blood. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AMICA/JAML

The 4E10 monoclonal antibody reacts with mouse JAML (junctional adhesion molecule-like protein), also known as AMICA. JAML is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAML is expressed on polymorphonuclear leukocytes (PMN), and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAML and epithelial coxsackie and adenovirus receptor (CAR). JAML is also expressed on lymphocyte subsets, such as gammadelta T cell lines, and can provide co-stimulatory signals for cell activation.

Long Name

Adhesion Molecule, Interacts With CXADR Antigen 1

Alternate Names

AMICA1, CREA7-1, JAML

Gene Symbol

JAML

Additional AMICA/JAML Products

Product Documents for AMICA/JAML Antibody (4E10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for AMICA/JAML Antibody (4E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AMICA/JAML Antibody (4E10) - BSA Free

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