AMPD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81269

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AMPD1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AEEKQIDDAMRNFAEKVFASEVKDEGGRQEISPFDVDEICPISHHEMQAHIFHLETLSTSTEARRKKRFQGRKTVNLSIPL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMPD1 Antibody - BSA Free

Western Blot: AMPD1 Antibody [NBP1-81269]

Western Blot: AMPD1 Antibody [NBP1-81269]

Western Blot: AMPD1 Antibody [NBP1-81269] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Skeletal muscle tissue
Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP1-81269]

Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP1-81269]

Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP1-81269] - Staining of human heart muscle shows low expression as expected.
Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP1-81269]

Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP1-81269]

Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP1-81269] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP1-81269]

Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP1-81269]

Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP1-81269] - Staining in human skeletal muscle and heart muscle tissues using anti-AMPD1 antibody. Corresponding AMPD1 RNA-seq data are presented for the same tissues.

Applications for AMPD1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPD1

Adenosine monophosphate deaminase 1 catalyzes the deamination of AMP to IMP in skeletal muscle and plays an important role in the purine nucleotide cycle. Two other genes have been identified, AMPD2 and AMPD3, for the liver- and erythocyte-specific isoforms, respectively. Deficiency of the muscle-specific enzyme is apparently a common cause of exercise-induced myopathy and probably the most common cause of metabolic myopathy in the human.

Alternate Names

adenosine monophosphate deaminase 1, adenosine monophosphate deaminase 1 (isoform M), adenosine monophosphate deaminase-1 (muscle), AMP deaminase 1, AMP deaminase isoform M, AMPD, EC 3.5.4.6, MAD, MADA, Myoadenylate deaminase, skeletal muscle AMPD

Gene Symbol

AMPD1

Additional AMPD1 Products

Product Documents for AMPD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for AMPD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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