Amphiphysin/AMPH Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32505

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Amphiphysin/AMPH Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Amphiphysin/AMPH. The exact sequence is proprietary.

Localization

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cytoplasm, cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Amphiphysin/AMPH Antibody - BSA Free

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.
Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded U87 xenograft. Antibody at dilution of 1:500.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505]

Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded RT2 xenograft; antibody dilution 1:500.
Amphiphysin/AMPH Antibody

Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] -

Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Amphiphysin/AMPH Antibody

Immunocytochemistry/ Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-32505] -

Immunocytochemistry/ Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-32505] - Amphiphysin/AMPH antibody [N1N2], N-term detects Amphiphysin/AMPH protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Amphiphysin/AMPH stained by Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Amphiphysin/AMPH Antibody

Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] -

Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Amphiphysin/AMPH Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Amphiphysin/AMPH

Amphiphysin, a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. Patient autoantibodies have a distinct pattern of reactivity with amphiphysin, and the dominant autoepitope is located in its C-terminal region, which contains an SH3 domain (1). Amphiphysin is expressed in many neurons, certain endocrine cell types, and spermatocytes (2). study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells (3).

Alternate Names

AMPH, AMPH1

Gene Symbol

AMPH

Additional Amphiphysin/AMPH Products

Product Documents for Amphiphysin/AMPH Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Amphiphysin/AMPH Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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