Angiopoietin-1 Antibody (S06-1F9)

Novus Biologicals | Catalog # NBP3-19492

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S06-1F9 expressed in HEK293
View all Angiopoietin-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Angiopoietin-1 (Uniprot # Q15389)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Angiopoietin-1 Antibody (S06-1F9)

Western Blot: Angiopoietin-1 Antibody (S06-1F9) [NBP3-19492]

Western Blot: Angiopoietin-1 Antibody (S06-1F9) [NBP3-19492]

Western Blot: Angiopoietin-1 Antibody (S06-1F9) [NBP3-19492] - Western blot detection of Angiopoietin-1 in Jurkat, C6, Hela cell lysates using NBP3-19492 (1:1000 diluted). Predicted band size: 58kDa. Observed band size: 75kDa.

Applications for Angiopoietin-1 Antibody (S06-1F9)

Application
Recommended Usage

Western Blot

1/2000-1/10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Angiopoietin-1

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Alternate Names

ANGPT1

Gene Symbol

ANGPT1

Additional Angiopoietin-1 Products

Product Documents for Angiopoietin-1 Antibody (S06-1F9)

Certificate of Analysis

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Product Specific Notices for Angiopoietin-1 Antibody (S06-1F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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