Angiopoietin-1 Antibody (SN67-02)

Novus Biologicals | Catalog # NBP2-67664

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN67-02 expressed in HEK293
View all Angiopoietin-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Angiopoietin-1 aa 249-300 / 498. (SwissProt: Q15389 Human; SwissProt: O35460 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Angiopoietin-1 Antibody (SN67-02)

Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664]

Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664]

Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664] - Analysis of Angiopoietin 1 on K562 cells lysates using anti-Angiopoietin 1 antibody at 1/1,000 dilution.
Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664]

Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664]

Western Blot: Angiopoietin-1 Antibody (SN67-02) [NBP2-67664] - Western blot analysis of Angiopoietin-1 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat

Applications for Angiopoietin-1 Antibody (SN67-02)

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Angiopoietin-1

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Alternate Names

ANGPT1

Gene Symbol

ANGPT1

Additional Angiopoietin-1 Products

Product Documents for Angiopoietin-1 Antibody (SN67-02)

Certificate of Analysis

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Product Specific Notices for Angiopoietin-1 Antibody (SN67-02)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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