Canine Angiopoietin-1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-42629

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma and other biological fluids (100 uL)

Sensitivity

0.63 ng/mL (example only; lot dependent)

Assay Range

1.56 - 100 ng/mL (example only; lot dependent)
View all Angiopoietin-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Canine

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Recovery for Canine Angiopoietin-1 ELISA Kit (Colorimetric)

Recovery

76 - 96% (example only; lot dependent)

Kit Contents for Canine Angiopoietin-1 ELISA Kit (Colorimetric)

  • Assay Diluent A
  • Assay Diluent B
  • Detection Reagent A
  • Detection Reagent B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated ready to use 96-well strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Angiopoietin-1

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Alternate Names

ANGPT1

Gene Symbol

ANGPT1

Additional Angiopoietin-1 Products

Product Documents for Canine Angiopoietin-1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Canine Angiopoietin-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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