ANKRD50 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-50031

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ANKRD50 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1200 and 1250 of human Ankyrin Repeat Domain 50 using the numbering given in entry NP_065070.1 (GeneID 57182).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANKRD50 Antibody - BSA Free

Western Blot: ANKRD50 Antibody [NBP1-50031]

Western Blot: ANKRD50 Antibody [NBP1-50031]

Western Blot: ANKRD50 Antibody [NBP1-50031] - Detection of Human and Mouse ANKRD50 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ANKRD50 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ANKRD50 was also immunoprecipitated by rabbit anti-ANKRD50 antibody which recognizes a downstream epitope. For blotting immunoprecipitated ANKRD50, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for ANKRD50 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ANKRD50

Alternate Names

ankyrin repeat domain 50

Gene Symbol

ANKRD50

Additional ANKRD50 Products

Product Documents for ANKRD50 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ANKRD50 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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