ANKRD50 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-50032

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Equine (100%), Guinea Pig (100%), Monkey (100%), Porcine (100%), Primate (100%), Rabbit (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ANKRD50 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1379 and 1429 of human Ankyrin Repeat Domain 50 using the numbering given in entry NP_065070.1 (GeneID 57182).

Reactivity Notes

Panda, Orangutan, Gorilla and Chimpanzee (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANKRD50 Antibody - BSA Free

Western Blot: ANKRD50 Antibody [NBP1-50032]

Western Blot: ANKRD50 Antibody [NBP1-50032]

Western Blot: ANKRD50 Antibody [NBP1-50032] - Detection of Human and Mouse ANKRD50 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ANKRD50 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ANKRD50 was also immunoprecipitated by rabbit anti-ANKRD50 antibody which recognizes an upstream epitope. For blotting immunoprecipitated ANKRD50, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for ANKRD50 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ANKRD50

Alternate Names

ankyrin repeat domain 50

Gene Symbol

ANKRD50

Additional ANKRD50 Products

Product Documents for ANKRD50 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ANKRD50 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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