Anti- Rituximab (Anti-Idiotype) Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
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Detection of Anti-MS4A1/CD20 (Rituximab) on Human MS4A1/CD20 transfectants by flow cytometry.
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Anti- Rituximab (Anti-Idiotype) Alexa Fluor® 647-conjugated Antibody Summary

Detects Rituximab in Western blots in direct ELISAs. 100% cross-reactivity with Tositumomab and Ibritumomab (95% or higher sequence identity with Rituximab) and no cross-reactivity with Cetuximab, Trastuzumab, Brentuximab, Gemtuzumab, Adalimumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Recombinant Monoclonal Rabbit IgG Clone # 2260A
Protein A or G purified from cell culture supernatant
Full-length Rituximab
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
HEK293 cell line transfected with Human CD20 and eGFP stained with Anti- Human CD20 (Rituximab), Catalog # MAB9575

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Anti-MS4A1/CD20 (Rituximab) on Human MS4A1/CD20 transfectants by flow cytometry. HEK293 human cell line transfected with (A) human MS4A1/CD20 or (B) irrelevant protein, and eGFP were stained with Human anti-Human MS4A1/CD20 (Rituximab) Monoclonal Antibody (MAB9575), then Alexa Fluor® 647-conjugated Anti-Rituximab (Anti-Idiotype) (Catalog # FAB9630R). Staining was performed using our Staining Membrane Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitution default not known
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Rituximab

The anti-Rituximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Rituximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats. In addition to Rituximab, the anti-Idiotype antibody also detects derivatives of Rituximab (Ibritumumab and Tositumumab).

Alternate Names
B lymphocyte antigen CD20; B lymphocyte surface antigen B1; Bp35; CD20; LEU 16; LEU16; Membrane spanning 4 domains subfamily A member 1; MS4A1; Rituximab

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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