Anti- Rituximab (Anti-Idiotype) PE-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Detection of Rituximab in PBMCs with CD19 costain cells by Flow Cytometry.
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Anti- Rituximab (Anti-Idiotype) PE-conjugated Antibody Summary

Detects Rituximab in Western blots in direct ELISAs. 100% cross-reactivity with Tositumomab and Ibritumomab (95% or higher sequence identity with Rituximab) and no cross-reactivity with Cetuximab, Trastuzumab, Brentuximab, Gemtuzumab, Adalimumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Recombinant Monoclonal Rabbit IgG Clone # 2260A
Protein A or G purified from cell culture supernatant
Full-length Rituximab
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Rituximab in PBMCs with CD19 costain cells by Flow Cytometry. PBMCs with CD19 costain were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Rabbit Anti-Rituximab (Anti-Idiotype) PE‑conjugated Monoclonal Antibody (Catalog # FAB9630P) or (B) Rabbit IgG PE-conjugated Antibody (Catalog # IC1051P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitution default not known
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Rituximab

The anti-Rituximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Rituximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats. In addition to Rituximab, the anti-Idiotype antibody also detects derivatives of Rituximab (Ibritumumab and Tositumumab).

Alternate Names
B lymphocyte antigen CD20; B lymphocyte surface antigen B1; Bp35; CD20; LEU 16; LEU16; Membrane spanning 4 domains subfamily A member 1; MS4A1; Rituximab

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Isotype Controls

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