AOF1 Antibody (JE32-40)

Novus Biologicals | Catalog # NBP3-32547

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE32-40 expressed in HEK293
View all AOF1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human AOF1 aa 71-120 / 822. (Uniprot: Q8NB78)

Localization

Nucleus, Chromosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AOF1 Antibody (JE32-40)

AOF1 Antibody (JE32-40)

Western Blot: AOF1 Antibody (JE32-40) [NBP3-32547]

Western Blot: AOF1 Antibody (JE32-40) [NBP3-32547] - Western blot analysis of AOF1 on different lysates with Rabbit anti-AOF1 antibody (NBP3-32547) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A549 cell lysate
Lane 4: K-562 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: U-2 OS cell lysate
Lane 7: HCT 116 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 92 kDa
Observed band size: 92 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32547) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for AOF1 Antibody (JE32-40)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AOF1

Long Name

Lysine-specific histone demethylase 1B

Alternate Names

C6orf193, EC 1.14.99.66, KDM1B, LSD2

Gene Symbol

KDM1B

Additional AOF1 Products

Product Documents for AOF1 Antibody (JE32-40)

Certificate of Analysis

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Product Specific Notices for AOF1 Antibody (JE32-40)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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