AP-2 beta/TFAP2B Antibody

Novus Biologicals | Catalog # NBP2-20606

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (99%), Mouse (100%), Porcine (99%), Xenopus (99%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all AP-2 beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human AP-2 beta/TFAP2B. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (99%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit AP-2 beta/TFAP2B Antibody (NBP2-20606) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for AP-2 beta/TFAP2B Antibody

Western Blot: AP-2 beta/TFAP2B Antibody [NBP2-20606]

Western Blot: AP-2 beta/TFAP2B Antibody [NBP2-20606]

Western Blot: AP-2 beta/TFAP2B Antibody [NBP2-20606] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:1000.
AP-2 beta/TFAP2B Antibody

Western Blot: AP-2 beta/TFAP2B Antibody [NBP2-20606] -

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AP2 beta antibody (NBP2-20606) diluted at 1:500.
AP-2 beta/TFAP2B Antibody

Immunoprecipitation: AP-2 beta/TFAP2B Antibody [NBP2-20606] -

AP2 beta antibody immunoprecipitates AP2 beta protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. 30 ug MCF-7 whole cell extract
B. Control with 4 ug of preimmune Rabbit IgG
C. Immunoprecipitation of AP2 beta protein by 4 ug AP2 beta antibody (NBP2-20606)
7.5 % SDS-PAGE
The immunoprecipitated AP2 beta protein was detected by AP2 beta antibody (NBP2-20606) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Applications for AP-2 beta/TFAP2B Antibody

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AP-2 beta

This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives. [provided by RefSeq]

Long Name

Activating Enhancer-binding Protein 2 beta

Alternate Names

AP2 beta, CHAR, TFAP2B

Gene Symbol

TFAP2B

UniProt

Q92481

Additional AP-2 beta Products

Product Documents for AP-2 beta/TFAP2B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for AP-2 beta/TFAP2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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