AP2 alpha Antibody (7D2B5) - BSA Free

Novus Biologicals | Catalog # NBP2-52572

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7D2B5

Format

BSA Free
View all AP2 alpha Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human AP2 alpha (AA: 1-100) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AP2 alpha Antibody (7D2B5) - BSA Free

Western Blot: AP2 alpha Antibody (7D2B5)BSA Free [NBP2-52572]

Western Blot: AP2 alpha Antibody (7D2B5)BSA Free [NBP2-52572]

Western Blot: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis using TFAP2A mAb against human TFAP2A (AA: 1-100) recombinant protein. (Expected MW is 37 kDa)
Immunohistochemistry-Paraffin: AP2 alpha Antibody (7D2B5) - BSA Free [NBP2-52572]

Immunohistochemistry-Paraffin: AP2 alpha Antibody (7D2B5) - BSA Free [NBP2-52572]

Immunohistochemistry-Paraffin: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of paraffin-embedded TFAP2A tissues using endometrial cancer mouse mAb with DAB staining.
Flow Cytometry: AP2 alpha Antibody (7D2B5) - BSA Free [NBP2-52572]

Flow Cytometry: AP2 alpha Antibody (7D2B5) - BSA Free [NBP2-52572]

Flow Cytometry: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of MCF-7 cells using TFAP2A mouse mAb (green) and negative control (red).
Western Blot: AP2 alpha Antibody (7D2B5)BSA Free [NBP2-52572]

Western Blot: AP2 alpha Antibody (7D2B5)BSA Free [NBP2-52572]

Western Blot: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis using TFAP2A mAb against HEK293 (1) and TFAP2A (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: AP2 alpha Antibody (7D2B5) - BSA Free [NBP2-52572]

Immunohistochemistry-Paraffin: AP2 alpha Antibody (7D2B5) - BSA Free [NBP2-52572]

Immunohistochemistry-Paraffin: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of paraffin-embedded ovarian cancer tissues using TFAP2A mouse mAb with DAB staining.
Flow Cytometry: AP2 alpha Antibody (7D2B5) - BSA Free [NBP2-52572]

Flow Cytometry: AP2 alpha Antibody (7D2B5) - BSA Free [NBP2-52572]

Flow Cytometry: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).
AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis using TFAP2A mouse mAb against Hela (1), MCF-7 (2), Cos7 (3), A431 (4), HCT116 (5), NIH/3T3 (6), and PC12 (7) cell lysate.
ELISA: AP2 alpha Antibody (7D2B5) - BSA Free [NBP2-52572]

ELISA: AP2 alpha Antibody (7D2B5) - BSA Free [NBP2-52572]

ELISA: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications for AP2 alpha Antibody (7D2B5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AP2 alpha

AP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.[supplied by OMIM]

Alternate Names

Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2 transcription factor, AP2-alpha, AP2TFAP-2alpha, AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha), BOFS, FLJ51761, TFAP2, transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha), transcription factor AP-2-alpha

Gene Symbol

TFAP2A

Additional AP2 alpha Products

Product Documents for AP2 alpha Antibody (7D2B5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for AP2 alpha Antibody (7D2B5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AP2 alpha Antibody (7D2B5) - BSA Free

Customer Reviews for AP2 alpha Antibody (7D2B5) - BSA Free

There are currently no reviews for this product. Be the first to review AP2 alpha Antibody (7D2B5) - BSA Free and earn rewards!

Have you used AP2 alpha Antibody (7D2B5) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies