AP2A2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92458

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all AP2A2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 590-939 of human Alpha2-adaptin/AP2A2 (NP_036437.1). STDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADLLGLGAAPPAPAGPPPSSGGSGLLVDVFSDSASVVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQTKPVDPTVEGGAQVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKEAVSQRLCELLSAQF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AP2A2 Antibody - Azide and BSA Free

Western Blot: AP2A2 AntibodyAzide and BSA Free [NBP2-92458]

Western Blot: AP2A2 AntibodyAzide and BSA Free [NBP2-92458]

Western Blot: AP2A2 Antibody [NBP2-92458] - Western blot analysis of extracts of various cell lines, using AP2A2 antibody (NBP2-92458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: AP2A2 AntibodyAzide and BSA Free [NBP2-92458]

Western Blot: AP2A2 AntibodyAzide and BSA Free [NBP2-92458]

Western Blot: AP2A2 Antibody [NBP2-92458] - Western blot analysis of extracts of SH-SY5Y cells, using AP2A2 antibody (NBP2-92458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
AP2A2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: AP2A2 Antibody - Azide and BSA Free [NBP2-92458] -

Immunocytochemistry/ Immunofluorescence: AP2A2 Antibody - Azide and BSA Free [NBP2-92458] - Immunofluorescence analysis of NIH/3T3 cells using AP2A2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
AP2A2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: AP2A2 Antibody - Azide and BSA Free [NBP2-92458] -

Immunocytochemistry/ Immunofluorescence: AP2A2 Antibody - Azide and BSA Free [NBP2-92458] - Immunofluorescence analysis of HeLa cells using AP2A2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Applications for AP2A2 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AP2A2

AP2A2 is a component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transportvia transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat componentsand appear to be involved in carg

Alternate Names

100 kDa coated vesicle protein C, Adapter-related protein complex 2 alpha-2 subunit, adaptin, alpha B, Adaptor protein complex AP-2 subunit alpha-2, adaptor-related protein complex 2, alpha 2 subunit, Alpha2-adaptin, Alpha-adaptin C, alpha-adaptin C; Huntingtin interacting protein J, AP-2 complex subunit alpha-2, Clathrin assembly protein complex 2 alpha-C large chain, clathrin-associated/assembly/adaptor protein, large, alpha 2, DKFZP564D1864, HIP9ADTAB, Huntingtin yeast partner J, Huntingtin-interacting protein 9, Huntingtin-interacting protein J, HYPJCLAPA2, KIAA0899HIP-9, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

Gene Symbol

AP2A2

Additional AP2A2 Products

Product Documents for AP2A2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AP2A2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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