AP3B2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81012

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AP3B2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: APVFMSENEFKKEQGKLMGMNEITEKLMLPDTCRSDHIVVQKVTATANLGRVPCGTSDEYRFAGRTLTGGSLVLLTLDARPAGAAQLTVNSEKMVIGTMLVKDVIQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AP3B2 Antibody - BSA Free

Western Blot: AP3B2 Antibody [NBP1-81012]

Western Blot: AP3B2 Antibody [NBP1-81012]

Western Blot: AP3B2 Antibody [NBP1-81012] - Analysis in control (vector only transfected HEK293T lysate) and AP3B2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012]

Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012]

Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012] - Staining in human cerebral cortex and pancreas tissues using anti-AP3B2 antibody. Corresponding AP3B2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012]

Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012]

Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012]

Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012]

Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012]

Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012]

Immunohistochemistry-Paraffin: AP3B2 Antibody [NBP1-81012] - Staining of human pancreas shows low expression as expected.

Applications for AP3B2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AP3B2

Adaptor protein-3 (AP3) is a heterotetrameric vesicle-coat protein complex. Some AP3 subunits are ubiquitously expressed, whereas others are expressed exclusively in neurons. The neuron-specific AP3 complex, which includes AP3B2, is thought to serve neuron-specific functions such as neurotransmitter release (Grabner et al., 2006 (PubMed 16788073)).(supplied by OMIM)

Alternate Names

Adapter-related protein complex 3 subunit beta-2, Adaptor protein complex AP-3 subunit beta-2, adaptor-related protein complex 3, beta 2 subunit, Beta-3B-adaptin, Clathrin assembly protein complex 3 beta-2 large chain, DKFZp686D17136, NAPTBAP-3 complex subunit beta-2, Neuronal adaptin-like protein, beta-subunit, Neuron-specific vesicle coat protein beta-NAP

Entrez Gene IDs

8120 (Human)

Gene Symbol

AP3B2

UniProt

Q13367 (Human)

Additional AP3B2 Products

Product Documents for AP3B2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for AP3B2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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