APE Antibody (S07-5A9)

Novus Biologicals | Catalog # NBP3-15113

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-5A9 expressed in HEK293
View all APE Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human APE (Uniprot#: P27695)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APE Antibody (S07-5A9)

Western Blot: APE Antibody (S07-5A9) [NBP3-15113]

Western Blot: APE Antibody (S07-5A9) [NBP3-15113]

Western Blot: APE Antibody (S07-5A9) [NBP3-15113] - Western blot detection of APE in K562, C6, 3T3, Hela cell lysates using APE Rabbit mAb (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 36kDa.

Applications for APE Antibody (S07-5A9)

Application
Recommended Usage

Western Blot

1/2000-1/10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APE

DNA-(apurinic or apyrimidinic site) lyase, also known as APE1 or APEX1, is a multifunctional mammalian proteins responsible for the cellular response to oxidative stress. Specifically, APE-1 both functions in the DNA base excision repair pathway and regulates the redox of transcriptional factors.

For DNA repair, APE1 functions as a mammalian apurinic/apyrimidinic (AP) endodeoxyribonuclease responsible for the repair of AP sites in DNA lesions induced by oxidative and alkylating agents.

APEX1 also exerts a reversible nuclear redox activity to regulate the DNA binding affinity and the activity of transcriptional factors by regulating the redox status of their DNA-binding domains.

APE1 antibodies are useful for studies on both DNA repair and transcriptional regulation.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Gene Symbol

APEX1

Additional APE Products

Product Documents for APE Antibody (S07-5A9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for APE Antibody (S07-5A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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