APOBEC3C Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35437

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all APOBEC3C Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human APOBEC3C (NP_055323.2).

Sequence:
MNPQIRNPMKAMYPGTFYFQFKNLWEANDRNETWLCFTVEGIKRRSVVSWKTGVFRNQVDSETHCHAERCFLSWFCDDILSPNTKYQVTWYTSWSPCPDCAGEVAEFLARHSNVNLTIFTARLYYFQYPCYQEGLRSLSQEGVAVEIMDYEDFKYCWENFVYNDNEPFKPWKGLKTNFRLLKRRLRESLQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APOBEC3C Antibody - BSA Free

APOBEC3C Antibody

Western Blot: APOBEC3C Antibody [NBP3-35437] -

Western Blot: APOBEC3C Antibody [NBP3-35437] - Western blot analysis of lysates from K-562 cells, using APOBEC3C Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
APOBEC3C Antibody

Immunoprecipitation: APOBEC3C Antibody [NBP3-35437] -

Immunoprecipitation: APOBEC3C Antibody [NBP3-35437] - Immunoprecipitation analysis of 200 ug extracts of K-562 cells, using 3 ug APOBEC3C antibody. Western blot was performed from the immunoprecipitate using APOBEC3C antibody at a dilution of 1:1000.

Applications for APOBEC3C Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APOBEC3C

APOBEC3C is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. [provided by RefSeq]

Alternate Names

APOBEC1LA3C, APOBEC1-like, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C, ARDC2, ARDC4, ARP5, bK150C2.3, EC 3.5.4.-, PBIMGC19485, Phorbolin I, probable DNA dC->dU-editing enzyme APOBEC-3C

Gene Symbol

APOBEC3C

Additional APOBEC3C Products

Product Documents for APOBEC3C Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for APOBEC3C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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