ARHGAP4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88527

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ARHGAP4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EHNGMRGLIPHKYITLPAGTEKQVVGAGLQTAGESGSSPEGLLASELVHRPEPCTSPEAMGPSGHRRRCLVPASPEQHVEVDKAVAQNMDSVFKELLGKTSVRQGLGPASTTSP

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARHGAP4 Antibody - BSA Free

ARHGAP4 Antibody - BSA Free Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Staining of human cerebral cortex, liver, lymphoid tissues and skeletal muscle using Anti-ARHGAP4 antibody NBP1-88527 (A) shows similar protein distribution across tissues to independent antibody NBP1-88528 (B).
ARHGAP4 Antibody - BSA Free Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Analysis in human spleen and skeletal muscle tissues using HPA001012 antibody. Corresponding ARHGAP4 RNA-seq data are presented for the same tissues.
ARHGAP4 Antibody - BSA Free Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp and white pulp.
ARHGAP4 Antibody - BSA Free Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.
ARHGAP4 Antibody - BSA Free Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Staining of human tonsil shows strong cytoplasmic positivity in germinal and non germinal center cells.
ARHGAP4 Antibody - BSA Free Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.
ARHGAP4 Antibody - BSA Free Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Staining of human cerebral cortex shows very weak cytoplasmic positivity in neuronal cells.
ARHGAP4 Antibody - BSA Free Western Blot: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Western Blot: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Analysis in human cell line RPMI-8226.
ARHGAP4 Antibody - BSA Free Western Blot: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Western Blot: ARHGAP4 Antibody - BSA Free [NBP1-88527]

Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Applications for ARHGAP4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARHGAP4

The ARHGAP4 gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-bindingproteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to theGolgi complex and can redi

Alternate Names

RGC1, Rho GTPase activating protein 4

Gene Symbol

ARHGAP4

Additional ARHGAP4 Products

Product Documents for ARHGAP4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ARHGAP4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARHGAP4 Antibody - BSA Free

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Protocols

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