ARHGEF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-46831

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ARHGEF1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 775 and 825 of human 115 kDa Rho guanine nucleotide exchange factor using the numbering given in entry NP_004697.2 (GeneID 9138).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARHGEF1 Antibody - BSA Free

Immunoprecipitation: ARHGEF1 Antibody [NBP1-46831]

Immunoprecipitation: ARHGEF1 Antibody [NBP1-46831]

Immunoprecipitation: ARHGEF1 Antibody [NBP1-46831] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-p115-RhoGEF antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. p115-RhoGEF was also immunoprecipitated by rabbit anti-p115-RhoGEF antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Applications for ARHGEF1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARHGEF1

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Alternate Names

115 kDa guanine nucleotide exchange factor, 115-kD protein, GEF1, LBCL2LSC, Lsc homolog, p115RhoGEF, p115-RhoGEF, Rho guanine nucleotide exchange factor (GEF) 1, rho guanine nucleotide exchange factor 1, SUB1.5

Gene Symbol

ARHGEF1

Additional ARHGEF1 Products

Product Documents for ARHGEF1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ARHGEF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ARHGEF1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review ARHGEF1 Antibody - BSA Free and earn rewards!

Have you used ARHGEF1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies