ARID1A Antibody (3611) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43566

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3611

Format

Azide and BSA Free
View all ARID1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID1A. The exact sequence is proprietary.

Reactivity Notes

Rat (81%), Bovine (84%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

242 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARID1A Antibody (3611) - Azide and BSA Free

Western Blot: ARID1A Antibody (3611) [NBP2-43566]

Western Blot: ARID1A Antibody (3611) [NBP2-43566]

Western Blot: ARID1A Antibody (3611) [NBP2-43566] - Analysis of whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody [3611] at a dilution of 1:500.
Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566]

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566]

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566] - Mouse colon. ARID1A stained by ARID1A antibody [3611] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566]

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566]

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566] - Mouse intestine. ARID1A stained by ARID1A antibody [3611] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566]

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566]

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566] - Human lung cancer. ARID1A stained by ARID1A antibody [3611] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ARID1A Antibody (3611) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ARID1A Antibody (3611) [NBP2-43566] -

ARID1A antibody [GT3611] detects ARID1A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ARID1A stained by ARID1A antibody [GT3611] (NBP2-43566) diluted at 1:200.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.

Applications for ARID1A Antibody (3611) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARID1A

ARID1A encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain ARID1A genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI- like), AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120SWI-like protein, BAF250A, BAF250SWI/SNF complex protein p270, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C10rf4, C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1, chromatin remodeling factor p250, hELD, hOSA1, matrix associated, actin dependent regulator of chromatin, Osa homolog 1, OSA1, OSA1 nuclear protein, P270, SMARCF1, subfamily f, member 1

Gene Symbol

ARID1A

Additional ARID1A Products

Product Documents for ARID1A Antibody (3611) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ARID1A Antibody (3611) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARID1A Antibody (3611) - Azide and BSA Free

Customer Reviews for ARID1A Antibody (3611) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review ARID1A Antibody (3611) - Azide and BSA Free and earn rewards!

Have you used ARID1A Antibody (3611) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies