Human Aromatase ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP2-69863
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
0.10 ng/mL
Assay Range
0.16 - 10 ng/mL
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Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Human
Specificity
This kit recognizes Human ARO in samples. No significant cross-reactivity or interference between Human ARO and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% 4.74%
Inter-Assay Precision (Precision between assays) CV% 5.71%
Recovery for Human Aromatase ELISA Kit (Colorimetric)
Recovery
84-109%
Linearity
Scientific Data Images for Human Aromatase ELISA Kit (Colorimetric)
ELISA: Human Aromatase ELISA Kit (Colorimetric) [NBP2-69863] -
ELISA: Human Aromatase ELISA Kit (Colorimetric) [NBP2-69863] - Standard Curve ReferenceKit Contents for Human Aromatase ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Aromatase
Aromatase is localized in the endoplasmic reticulum of the cell and its activity is regulated by tissue specific promoters that are in turn controlled by hormones, cytokines, and other factors. It can be found in many tissues reproductive tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, endometrium as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer.
Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function. The activity is increased by environmental factors such as age, obesity, insulin, gonadotropins, and alcohol but decreased by prolactin, anti-m llerian hormone, and smoking.
Long Name
Aromatase
Alternate Names
ARO1, CYAR, CYP19, CYP19A1, CYPXIX, EC 1.14.14.14, Estrogen synthase
Gene Symbol
CYP19A1
Additional Aromatase Products
Product Documents for Human Aromatase ELISA Kit (Colorimetric)
Certificate of Analysis
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Product Specific Notices for Human Aromatase ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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