The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in intestinal brush border membranes, but is also expressed in the kidney, brain and liver. ASAH2 is involved in the metabolism of dietary sphingolipids and the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.
ASAH2/N-acylsphingosine Amidohydrolase-2 Recombinant Protein Antigen
Novus Biologicals | Catalog # NBP2-49326PEP
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Key Product Details
Source
E. coli
Applications
Antibody Competition
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Product Specifications
Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human ASAH2/N-acylsphingosine Amidohydrolase-2.
Source: E. coli
Amino Acid Sequence: RTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQNHQT
Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)
This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Purity
>80% by SDS-PAGE and Coomassie blue staining
Predicted Molecular Mass
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications
Antibody Competition (10 - 100 molar excess)
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-49326.
It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.
For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com
Protein / Peptide Type
Recombinant Protein Antigen
Formulation, Preparation, and Storage
NBP2-49326PEP
| Formulation | PBS and 1M Urea, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Background: ASAH2/N-acylsphingosine Amidohydrolase-2
Long Name
Neutral Ceramidase
Alternate Names
Nacylsphingosine Amidohydrolase2
Gene Symbol
ASAH2
Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products
Product Documents for ASAH2/N-acylsphingosine Amidohydrolase-2 Recombinant Protein Antigen
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ASAH2/N-acylsphingosine Amidohydrolase-2 Recombinant Protein Antigen
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
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