Aspartate Aminotransferase Antibody (5J1B4)

Novus Biologicals | Catalog # NBP3-15347

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5J1B4 expressed in HEK293
View all Aspartate Aminotransferase Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Aspartate Aminotransferase (NP_002070.1). AAGFKDIRSYRYWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFVSEGFE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aspartate Aminotransferase Antibody (5J1B4)

Western Blot: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347]

Western Blot: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347]

Western Blot: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347] - Western blot analysis of extracts of various cell lines, using Aspartate Aminotransferase Rabbit mAb (NBP3-15347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347]

Western Blot: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347]

Western Blot: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347] - Western blot analysis of extracts of various cell lines, using Aspartate Aminotransferase Rabbit mAb (NBP3-15347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Aspartate Aminotransferase Antibody (5J1B4)

Immunocytochemistry/ Immunofluorescence: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347] -

Immunocytochemistry/ Immunofluorescence: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347] - Confocal imaging of HeLa cells using Aspartate Aminotransferase Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Applications for Aspartate Aminotransferase Antibody (5J1B4)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aspartate Aminotransferase

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Long Name

Glutamic-oxaloacetic transaminase 1

Alternate Names

aspartate aminotransferase, cytoplasmic, AST1, ASTQTL1, CASPAT, CCAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, EC 2.6.1.1, EC 2.6.1.3, GIG18, Glutamate oxaloacetate transaminase 1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GOT1, growth-inhibiting protein 18, SGOT, Transaminase A

Gene Symbol

GOT1

Additional Aspartate Aminotransferase Products

Product Documents for Aspartate Aminotransferase Antibody (5J1B4)

Certificate of Analysis

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Product Specific Notices for Aspartate Aminotransferase Antibody (5J1B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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