Recombinant Human Aspartate Aminotransferase His Protein

Name: Recombinant Human Aspartate Aminotransferase His Protein
Brand: Novus Biologicals
SKU: NBP2-52179
Price: 219 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-52179

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE

View all Aspartate Aminotransferase Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-413 of Human Aspartate Aminotransferase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

48.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Aspartate Aminotransferase His Protein

SDS-PAGE: Recombinant Human Aspartate Aminotransferase His Protein [NBP2-52179]

SDS-Page: Recombinant Human Aspartate Aminotransferase Protein [NBP2-52179] - 15% SDS Page (3 ug)

Formulation, Preparation, and Storage

NBP2-52179

Formulation	20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 2 mM DTT, 100 mM NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aspartate Aminotransferase

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Long Name

Glutamic-oxaloacetic transaminase 1

Alternate Names

aspartate aminotransferase, cytoplasmic, AST1, ASTQTL1, CASPAT, CCAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, EC 2.6.1.1, EC 2.6.1.3, GIG18, Glutamate oxaloacetate transaminase 1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GOT1, growth-inhibiting protein 18, SGOT, Transaminase A

Gene Symbol

GOT1

Additional Aspartate Aminotransferase Products

Product Documents for Recombinant Human Aspartate Aminotransferase His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Aspartate Aminotransferase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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