Recombinant Human Aspartate beta hydroxylase His Protein

Name: Recombinant Human Aspartate beta hydroxylase His Protein
Brand: Novus Biologicals
SKU: NBP1-40403
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-40403

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all Aspartate beta hydroxylase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 75-270 of Human Aspartate beta hydroxylase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MFDLVDYEEV LGKLGIYDAD GDGDFDVDDA KVLLGLKERS TSEPAVPPEE AEPHTEPEEQ VPVEAEPQNI EDEAKEQIQS LLHEMVHAEH ETEHSYHVEE TVSQDCNQDM EEMMSEQENP DSSEPVVEDE RLHHDTDDVT YQVYEEQAVY EPLENEGIEI TEVTAPPEDN PVEDSQVIVE EVSIFPVEEQ QEVPPDT

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

24.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Aspartate beta hydroxylase His Protein

SDS-PAGE: Recombinant Human Aspartate beta hydroxylase His Protein [NBP1-40403]

SDS-Page: Recombinant Human Aspartate beta hydroxylase Protein [NBP1-40403] - ASPH, 24.5 kDa (217aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation, and Storage

NBP1-40403

Formulation	20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aspartate beta hydroxylase

ASPH (Aspartate beta-hydroxylase) is a widely-expressed type II membrane protein involved in calcium homeostasis. Located in the endoplasmic reticulum, ASPH specifically hydroxylates an Asp or Asn residue in the epidermal growth factor-like (EGF) domains of several proteins, using iron as a cofactor. While all ASPH variants are expressed in skeletal muscle, only some are detected in heart, brain, pancreas, placenta, lung, liver, and kidney tissues. Recombinant human ASPH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

AAH, ASP beta-hydroxylase, aspartate beta-hydroxylaseA beta H-J-J, aspartyl/asparaginyl-beta-hydroxylase, BAH, cardiac junctin, CASQ2BP1, EC 1.14.11.16, HAAHaspartyl/asparaginyl beta-hydroxylase, humbug, JCTN, junctate, junctin, Peptide-aspartate beta-dioxygenase

Gene Symbol

ASPH

Additional Aspartate beta hydroxylase Products

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Aspartate beta hydroxylase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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