ASTE1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81661

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ASTE1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CFVQCFSEADRDIMTLANHWNCPVLSSDSDFCIFDLKTGFCPLNSFQWRNMNTIKGTQNYIPAKCFSLDAFCHHFSNMNKALLPLFAVLCGNDHVNLPIME

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASTE1 Antibody - BSA Free

Western Blot: ASTE1 Antibody [NBP1-81661]

Western Blot: ASTE1 Antibody [NBP1-81661]

Western Blot: ASTE1 Antibody [NBP1-81661] - Analysis in control (vector only transfected HEK293T lysate) and ASTE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ASTE1 Antibody [NBP1-81661]

Immunocytochemistry/ Immunofluorescence: ASTE1 Antibody [NBP1-81661]

Immunocytochemistry/Immunofluorescence: ASTE1 Antibody [NBP1-81661] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
ASTE1 Antibody - BSA Free Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
ASTE1 Antibody - BSA Free Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Staining of human testis shows strong cytoplasmic positivity in Leydig cells.
ASTE1 Antibody - BSA Free Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Staining of human placenta shows weak cytoplasmic positivity in trophoblastic cells.
ASTE1 Antibody - BSA Free Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Staining of human prostate shows strong cytoplasmic positivity in basal cells.
ASTE1 Antibody - BSA Free Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Staining of human epididymis shows strong cytoplasmic positivity in basal cells.

Applications for ASTE1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASTE1

ASTE1, also known as Protein asteroid homolog 1, is a 679 amino acid protein that is 77 kDa, belongs to the asteroid family, and has a possible role in EGF receptor signaling. The protein is being studied for its involvement in colorectal cancer. ASTE1 has been linked to ERBB3 in DNA repair pathway.

Alternate Names

asteroid homolog 1 (Drosophila), HT001, MGC129980, protein asteroid homolog 1

Gene Symbol

ASTE1

Additional ASTE1 Products

Product Documents for ASTE1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ASTE1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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