ATF5 Antibody (9D8) - BSA Free

Novus Biologicals | Catalog # NBP3-26496

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9D8 expressed in HEK293

Format

BSA Free
View all ATF5 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human ATF5 [UniProt Q9Y2D1]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATF5 Antibody (9D8) - BSA Free

ATF5 Antibody (9D8)

Immunohistochemistry: ATF5 Antibody (9D8) [NBP3-26496] -

Immunohistochemistry: ATF5 Antibody (9D8) [NBP3-26496] - Image of ATF5 Antibody (9D8) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
ATF5 Antibody (9D8)

Western Blot: ATF5 Antibody (9D8) [NBP3-26496] -

Western Blot: ATF5 Antibody (9D8) [NBP3-26496] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue.
All lanes: ATF5 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 31 kDa
Observed band size: 35 kDa

Applications for ATF5 Antibody (9D8) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: ATF5

Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4

Alternate Names

activating transcription factor 5FLJ34666, ATFXHMFN0395, cAMP-dependent transcription factor ATF-5, cyclic AMP-dependent transcription factor ATF-5, Transcription factor ATFx

Gene Symbol

ATF5

Additional ATF5 Products

Product Documents for ATF5 Antibody (9D8) - BSA Free

Certificate of Analysis

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Product Specific Notices for ATF5 Antibody (9D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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