ATP6V1C1 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-99458
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Cynomolgus Monkey (100%), Orangutan (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ATP6V1C1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ATP6V1C1 Antibody - Azide and BSA Free
Western Blot: ATP6V1C1 Antibody [NBP2-99458]
Western Blot: ATP6V1C1 Antibody [NBP2-99458] - Anti-ATP6V1C1 rabbit polyclonal antibody at 1:10000 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDaImmunoprecipitation: ATP6V1C1 Antibody [NBP2-99458]
Immunoprecipitation: ATP6V1C1 Antibody [NBP2-99458] - ATP6V1C1 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 4 ul anti-ATP6V1C1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ATP6V1C1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 50 kDaApplications for ATP6V1C1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATP6V1C1
Alternate Names
ATP6CH+-transporting ATPase chain C, vacuolar, ATP6Dsubunit C of vacuolar proton-ATPase V1 domain, ATPase, H+ transporting, lysosomal (vacuolar proton pump) 42kD, ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C, isoform 1, ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1, FLJ20057, H(+)-transporting two-sector ATPase, subunit C, vacuolar ATP synthase subunit C, vacuolar proton pump C subunit, Vacuolar proton pump subunit C 1, vacuolar proton pump, 42-kD subunit, vacuolar proton-ATPase, subunit C, VI domain, VATCH+ -ATPase C subunit, V-ATPase C subunit, V-ATPase subunit C 1, Vma5, V-type proton ATPase subunit C 1
Gene Symbol
ATP6V1C1
Additional ATP6V1C1 Products
Product Documents for ATP6V1C1 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ATP6V1C1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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