ATP7b Antibody (S62-29) - BSA Free

Novus Biologicals | Catalog # NBP2-59375

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S62-29

Format

BSA Free
View all ATP7b Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide amino acids 3-21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2

Localization

Cytoplasm, Mitochondrion, Golgi Apparatus, Trans-Golgi Network Membrane

Specificity

Detects 160kDa in rat brain membrane preparations.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ATP7b Antibody (S62-29) - BSA Free

Western Blot: ATP7b Antibody (S62-29) [NBP2-59375]

Western Blot: ATP7b Antibody (S62-29) [NBP2-59375]

Western Blot: ATP7b Antibody (S62-29) [NBP2-59375] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.
Immunocytochemistry/ Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375]

Immunocytochemistry/ Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375]

Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 2 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375]

Immunocytochemistry/ Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375]

Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X.
Immunocytochemistry/ Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375]

Immunocytochemistry/ Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375]

Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Tissue: NIH 3T3 (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Copper Transporting ATPase 2 Antibody. (D) Composite.

Applications for ATP7b Antibody (S62-29) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: ATP7b

ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; form A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver.

Alternate Names

ATPase, Cu(2+)- transporting, beta polypeptide, ATPase, Cu++ transporting, beta polypeptide, Copper pump 2, copper-transporting ATPase 2, EC 3.6.3, EC 3.6.3.4, PWD, WC1, WD, Wilson disease-associated protein, WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Gene Symbol

ATP7B

Additional ATP7b Products

Product Documents for ATP7b Antibody (S62-29) - BSA Free

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Product Specific Notices for ATP7b Antibody (S62-29) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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