ATRX Antibody (4212) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43599

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4212

Format

Azide and BSA Free
View all ATRX Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATRX. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chimpanzee (100%), Mouse (89%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

283 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATRX Antibody (4212) - Azide and BSA Free

Western Blot: ATRX Antibody (4212) [NBP2-43599]

Western Blot: ATRX Antibody (4212) [NBP2-43599]

Western Blot: ATRX Antibody (4212) [NBP2-43599] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [GT4212].
Western Blot: ATRX Antibody (4212) [NBP2-43599]

Western Blot: ATRX Antibody (4212) [NBP2-43599]

Western Blot: ATRX Antibody (4212) [NBP2-43599] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug BCL-1 whole cell lysate/extract 5 % SDS-PAGE ATRX antibody [4212] dilution: 1:500.
ATRX Antibody (4212) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ATRX Antibody (4212) [NBP2-43599] -

ATRX antibody [GT4212] detects ATRX protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATRX protein stained by ATRX antibody [GT4212] (NBP2-43599) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for ATRX Antibody (4212) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATRX

ATRX is encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.

Alternate Names

alpha thalassemia/mental retardation syndrome X-linked, alpha thalassemia/mental retardation syndrome X-linked (RAD54 (S. cerevisiae)homolog), alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S.cerevisiae), ATP-dependent helicase ATRX, ATR2, DNA dependent ATPase and helicase, EC 3.6.1, EC 3.6.4.12, helicase 2, X-linked, Juberg-Marsidi syndrome, MGC2094, MRXHF1, RAD54 homolog, RAD54L, SFM1, SHS, transcriptional regulator ATRX, XH2RAD54, X-linked helicase II, X-linked nuclear protein, XNPZNF-HX, Zinc finger helicase, Znf-HX

Gene Symbol

ATRX

Additional ATRX Products

Product Documents for ATRX Antibody (4212) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ATRX Antibody (4212) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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