Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP2-66682

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL
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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

This kit recognizes Human AIRE in samples. No significant cross-reactivity or interference between Human AIRE and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 9.66%

Inter-Assay Precision (Precision between assays) CV% 9.77%

Recovery for Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

Recovery

91-106%

Linearity

Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

Scientific Data Images for Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

ELISA: Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence) [NBP2-66682] -

ELISA: Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence) [NBP2-66682] - Standard Curve Reference

Kit Contents for Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Autoimmune Regulator/AIRE

AIRE encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Alternate Names

AIRE, APECED, APS1, APSI, PGA1

Gene Symbol

AIRE

Additional Autoimmune Regulator/AIRE Products

Product Documents for Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

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  • Q: I am looking into a positive control for Western blotting. We are trying to detect the AIRE protein and are not sure whether the antibody we have is picking up the protein. Are you able to tell me whether the human thymus whole tissue lysate (NB820-59456) has been used to detect for AIRE expression by Western blotting?

    A:

    I am not currently aware of any references to the use of our human thymus whole tissue lysate being used to detect AIRE by Western blotting. On looking through the Western blot images for all seven of our AIRE antibodies, I see that these have been generated using lysates from RT-4 cells (bladder), U-251 MG cells (brain), 293 cells (human embryonic kidney), human spleen lysate, liver lysate and tonsil lysate. Additionally, you may find the Human Protein Atlas link to AIRE useful. At this site you can find protein expression profiles based on immunohistochemistry for a large number of human tissues, cancers and cell lines: The Human Protein Atlas.

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